Chiral Columns

CHIRAL TECHNOLOGIES, INC. has expanded its portfolio of the well-known Daicel columns by adding chiral protein stationary phases: CHIRALPAK® AGP, CHIRALPAK HSA and CHIRALPAK CBH. The chiral selector for the CHIRALPAK AGP is α1-acid glycoprotein (AGP), while human serum albumin (HSA) is the selector for CHIRALPAK HSA. The chiral selector for CHIRALPAK CBH is cellobiohydrolase, a stable enzyme. The three chiral selectors are immobilized on 5-µm spherical silica particles.

The protein stationary phases (PSPs) were originally developed and manufactured by ChromTech Ltd, U.K. Chiral Technologies Europe acquired ChromTech in 2009 and we are now the only manufacturer of these widely-recognized PSPs and columns.
The PSPs function entirely in the reversed-phase chromatographic mode, using buffers with low organic modifier content and at moderate pH values. A vast variety of chiral compounds can be separated using the PSP-based columns. Click here to download our brochure.
The CHIRALPAK AGP selector has extremely broad applicability for the separation of chiral drugs. The CHIRALPAK CBH is an effective complement to the CHIRALPAK AGP for separation of basic drugs of many compound types, while the CHIRALPAK HSA can be used to separate acidic compounds directly, without derivatization prior to analysis, and also nonprotolytic compounds. The mobile phases, with which these columns are used, are highly compatible with MS and MS/MS analyses and a number of literature papers demonstrate their utility in HPLC separations and detection.
Enantioselectivity can be easily controlled or improved by changes in the mobile phase composition: pH, buffer and organic modifier types and concentrations.
The PSP columns are successfully applied to enantiomeric purity analyses of bulk drugs and also of finished drug formulations. The United States Pharmacopeia (USP), the widely-used and continuously-revised compendium of standardized testing methods, lists the CHIRALPAK AGP brand of L41 column for the HPLC methods to test Enantiomeric Purity of the single-enantiomer and racemic drugs.
Chromatograms below show some examples of drug separations.
Another exciting area of applications of the protein-based columns is their use in drug-protein binding studies. The degree of drug-protein binding directly affects pharmacokinetic and pharmacodynamic characteristics of a drug. Drug-protein binding is the reversible interaction of drugs with proteins in plasma. A drug's potency, therefore, may be dependent on the degree to which it binds to the plasma proteins and other blood constituents. Major blood proteins to which drugs bind are human serum albumin, α1-acid glycoprotein, lipoproteins and globulins.

Although a number of methods are available to measure the degree of the drug interaction with proteins in plasma, HPLC is also a convenient and fast way to determine the drug-protein binding properties. Both CHIRALPAK AGP and CHIRALPAK HSA columns have been successfully used for these studies.

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